If you were looking for a different "C18zip" (such as a specific software tool or a different technical manual), please share a few more details so I can find the right text for you!
Dispense the liquid into a clean, sterile microcentrifuge tube.
💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry.
50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste.
Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials
Aspirate and dispense your peptide sample through the ZipTip.
Repeat using the same elution volume to ensure all peptides are recovered.
C18zip Apr 2026
If you were looking for a different "C18zip" (such as a specific software tool or a different technical manual), please share a few more details so I can find the right text for you!
Dispense the liquid into a clean, sterile microcentrifuge tube. C18zip
💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry. If you were looking for a different "C18zip"
50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste. 50–70% ACN in 0
Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials
Aspirate and dispense your peptide sample through the ZipTip.
Repeat using the same elution volume to ensure all peptides are recovered.