Guidelines for the use of flow cytometry and cell sorting in immunological studies - PMC. PubMed Central (PMC) (.gov)

: Researchers shine light at two different wavelengths (typically : Light increases when calcium is present. : Light decreases when calcium is present. The Ratio : By calculating the

: Always keep your Fura-2 samples in the dark (wrapped in foil) as much as possible, as the dye will "bleach" and stop working if exposed to too much light.

Guidelines for the use of flow cytometry and cell sorting ... - PMC